Several of these 286 RNAs were shown to belong to the same transcriptional units in S. The presence of 22 RNAs within HG003 EVs was confirmed by reverse transcription quantitative PCR (RT-qPCR) experiments. Twenty-eight sRNAs were also detected, including bona fide RsaC. They coded for numerous virulence-associated factors ( hld encoded by the multifunctional sRNA RNAIII, agrBCD, psmβ1, sbi, spa, and isaB), ribosomal proteins, transcriptional regulators, and metabolic enzymes.
They corresponded to 286 unique RNAs, including 220 mRNAs. According to growth conditions, from 86 to 273 highly covered RNAs were identified into the EVs. However, only ~5% of them were highly covered by reads (≥90% coverage) indicating that a large fraction of EV RNAs, notably mRNAs and sRNAs, were fragmented in EVs.
On average, sequences corresponding to 78.0% of the annotated transcripts in HG003 genome were identified in HG003 EVs. aureus strain HG003 under different environmental conditions: early- and late-stationary growth phases, and presence or absence of a sublethal vancomycin concentration. A high-throughput RNA sequencing approach identified RNAs in EVs produced by the clinical S. Here, we investigated in depth the RNA content of S. Staphylococcus aureus, an opportunistic human and animal pathogen, produces EVs whose RNA content is still poorly characterized. Bacterial EVs contain RNA, which may serve in communicating with their infected hosts. These membranous spheres released by many living cells carry various macromolecules, some of which influence host-pathogen interactions. The roles of bacterial extracellular vesicles (EVs) in cell-to-cell signaling are progressively being unraveled. 3BRM UMR_S 1230, University of Rennes, Inserm, Rennes, France.2Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.1INRAE, Institut Agro, STLO, Rennes, France.Brenda Silva Rosa Da Luz 1,2, Aurélie Nicolas 1, Svetlana Chabelskaya 3, Vinícius de Rezende Rodovalho 1,2, Yves Le Loir 1, Vasco Ariston de Carvalho Azevedo 2, Brice Felden 3 * † and Eric Guédon 1 * †
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